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Question 1 (a) 'n Mutante vorm van die polipeptiedhormoon-(Answered)

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Question 1

(a) ?n Mutante vorm van die polipeptiedhormoon angiotensien II wat verhoogde bloeddruk en vloeistof-retensie veroorsaak het die volgende aminosuursamestelling: /

A mutant form of the polypeptide hormone angiotensin II, which causes hypertension and fluid retention has the following amino acid composition:

Val, Tyr, Pro, Phe, Met, Ile, Arg, Asp

Lei die aminosuurvolgorde van die hormoon af van die volgende data. /

Deduce the amino acid sequence of the hormone from the following data.

? Tripsienvertering van die hormoon gee ?n dipeptied wat Arg en Asp bevat en ?n heksapeptied wat die res van die aminosure bevat. /

Trypsin digestion of the hormone yields a dipeptide containing Arg and Asp and a hexapeptide containing the rest of the amino acids.

? Sianogeenbromied-klowing van die hormoon gee ?n dipeptied wat Phe en Pro bevat en ?n heksapeptied wat die res van die aminosure bevat. /

Cyanogen bromide cleavage of the hormone yields a dipeptide containing Phe and Pro and a hexapeptide containing the rest of the amino acids.

? Kimotripsienvertering kloof die hormoon in 2 tetrapeptiede met die volgende aminosuursamestellings: /

Chymotrypsin digestion cleaves the hormone into 2 tetrapeptides with the following amino acid compositions:

(Asp, Arg, Tyr, Val) en/and (Ile, Met, Phe, Pro)

Question 2

?n Dekapeptied het die volgende eienskappe: /

A decapeptide has the following characteristics:

? Die aminosuursamestelling is: /

The amino acid composition is:

His, Ala, Tyr, Arg, Asp, Gly, Glu, Lys, Met, Pro

? N-terminale aminosuurbepaling met fenielisothiosianaat gee PTH-glisien. /

N-terminal amino acid determination with phenylisothiocyanate yields PTH-glycine.

? Tripsienvertering gee 3 peptiede met die volgende aminosuursamestellings: /

Trypsin digestion yields 3 peptides with the amino acid compositions:

T-1: Asp, Glu, Lys, Pro, Ala

T-2: Gly, Arg, His

T-3: Met, Tyr

? Edman se reagens gee met peptied T-1 na siklusse 1 en 2: Pro en Ala respektiewelik. /

Edman?s reagent yields with peptide T-1 after cycles 1 and 2: Pro and Ala respectively.

? Chimotripsienvertering gee 2 fragmente met die aminosuursamestellings: /

Chymotrypsin digestion yields 2 fragments with amino acid compositions:

C-1: Gly, Asp, Arg, Lys, Glu, Tyr, Pro, Ala, His

C-2: metionien / methionine

? S. aureus V8 vertering gee 2 fragmente met aminosuursamestellings: /

S. aureus V8 digestion yields 2 fragments with amino acid compositions:

Sa 1: Asp, Tyr, Lys, Met

Sa 2: Ala, Gly, Glu, Arg, Pro, His

Lei die aminosuurvolgorde van die dekapeptied af. /

Deduce the amino acid sequence of the decapeptide.

 

Paper#9210642 | Written in 27-Jul-2016

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